Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: Primer sequences

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques:

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: Immunohistochemistry. (A and B) show positive expression of PKM2 and NOTCH1 in colorectal cancer tissue, while (C and D) show no expression of the two in normal tissue (×200).

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques: Immunohistochemistry, Expressing

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: Analysis of correlation between PKM2 and NOTCH1 in colorectal cancer tissue.

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques:

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: Relationship between PKM2 and NOTCH1 expressions and clinical features of colorectal cancer

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques: Marker

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: Cox regression analysis

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques:

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: NOTCH1 and PKM2 expressions in colorectal cancer cells. A, B. qRT-PCR was utilized to determine mRNA levels of PKM2 and NOTCH1 in colorectal cancer cells. C-F. Western blot was utilized to determine the protein levels of PKM2 and NOTCH1 in colorectal cancer cells. ***P<0.001; **P<0.01; ****P<0.0001.

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques: Quantitative RT-PCR, Western Blot

Journal: American Journal of Translational Research

Article Title: Down-regulation of NOTCH1 and PKM2 can inhibit the growth and metastasis of colorectal cancer cells

doi:

Figure Lengend Snippet: PKM2 and NOTCH1 expressions in colorectal cancer cells treated with compounds 3K and TGN. A-D. qRT-PCR was carried out for measuring mRNA levels of PKM2 and NOTCH1 in the 4 groups of colorectal cancer cells after 24-h incubation with DMSO (as a control), compounds 3K, TGN, or the combination of compound 3K and TGN. E-J. Western blot was conducted to determine the protein levels of PKM2 and NOTCH1 in the 4 groups after 24 h incubation. *P<0.05; **P<0.01; ***P<0.001, D, DMSO; P, Compound 3K; N, tangeretin; P+N, Compound 3K+tangeretin.

Article Snippet: Afterwards, the sections were subjected to 16-h incubation (4°C) with primary antibodies against PKM2 and NOTCH1 and then 2-h incubation (indoor temperature) with horseradish peroxidase (HRP)-conjugated secondary antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, CN).

Techniques: Quantitative RT-PCR, Incubation, Control, Western Blot

Journal: The Journal of Neuroscience

Article Title: Increased Generation of Neuronal Progenitors after Ischemic Injury in the Aged Adult Human Forebrain

doi: 10.1523/JNEUROSCI.4667-06.2006

Figure Lengend Snippet: Cellular architecture and proliferative capacity of the adult human SVZ. a, Schematic overview of a human brain hemisphere with the dotted red line indicating the area of analysis. b, c, Representative histology of the SVZ with a gap between the ependymal layer and astrocytic ribbon in the medial part (b) and with the astrocytic ribbon being continuous from the ependymal layer to the brain parenchyma in the dorsal and ventral areas (c). d, Occasionally, PSA-NCAM-positive cells can be detected close to the ependyma or in the astrocytic ribbon and beyond. e, f, Molecules that were shown to regulate neurogenesis such as notch1 (e) and Flk-1/VEGFR-2 (f) are expressed in the ependymal cell layer. g, Proliferating Ki-67-positive cells are often closely associated with the ependymal layer. h, i, Lack of costaining with pan-hematopoietic markers such as CD45 (h) rules out a non-neural origin of the proliferating Ki-67-positive cells (i). Scale bars: b, c, 50 μm; d, g, h, 10 μm; e, f, 25 μm; i, 20 μm.

Article Snippet: The following antibodies were used: mouse anti-β-III-tubulin (clone Tuj1 1:500; Covance), monoclonal mouse anti-polysialic acid neural cell adhesion molecule (PSA-NCAM, clone 2–2B, 1:2000; Millipore, Temecula, CA), monoclonal mouse anti-human notch1 (1:2000, Ab-1, clone A6; Biocarta Europe, Hamburg, Germany), polyclonal rabbit anti-vascular endothelial growth factor -receptor-2 (VEGFR2)/fetal liver kinase receptor 1 (Flk-1) (1:100; Zytomed, Berlin, Germany), monoclonal mouse anti-glial fibrillary acidic protein (GFAP, 1:500; Millipore), polyclonal rabbit anti-GFAP (1:1000; Dako, Carpinteria, CA), monoclonal mouse anti-human CD45/leukocyte common antigen (1:50, clones 2B11+PD7/26; Dako), monoclonal mouse anti-human CD68 (clone PG-M1, 1:100; Dako), monoclonal mouse anti-human CD34 (clone QBEND10, 1:100; Immunotech, Marseille, France), polyclonal rabbit anti-human S100β (1:100; Dako), monoclonal mouse anti-human nestin (1:200; Millipore), polyclonal rabbit anti-Mash1 (1:100; Millipore), polyclonal rabbit anti-Musashi (1:100; Millipore), and monoclonal mouse anti-human Ki-67 (clone MIB-1, 1:50; Dako).

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